Microscopic Cellular Structure

: Breast Tissue Regeneration using Adipose Derived Mesenchymal Stem cell This work package aims to develop a tissue engineering approach for breast regeneration utilizing adipose-derived mesenchymal stem cells (AD-MSCs) seeded onto methylcellulose hydrogel scaffolds. The necessity of scaffold permeability for adipose tissue growth suggests that factors crucial for angiogenesis and cellular proliferation, such as vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and tumor necrosis factor-α (TNF-α), along with essential nutrients, are present outside the tissue engineering construct (TEC). Furthermore, insights from mammary gland development are integral to this work package. The natural process of mammary gland development involves intricate cellular interactions, including the proliferation and differentiation of epithelial and adipose tissue compartments